c57bl 6 cd45 2 wild type  (Jackson Laboratory)

Journal: Nature Communications

Article Title: Aiolos restricts the generation of antigen-inexperienced, virtual memory CD8 + T cells in mice

doi: 10.1038/s41467-025-67540-8

Figure Lengend Snippet: Flow cytometry analysis of CD8 + T cells from the spleens of uninfected WT and Ikzf3 -/- mice. Mice were injected with fluorophore (PE) labeled anti-CD45.2 antibody via the retro-orbital route to distinguish resident (CD45.2 (i.v. Neg) ) from circulating (CD45.2 (i.v. Pos) ) T VM cells. a Representative flow contour plots depicting CD8 + T VM (CD62L + CD44 + CD49d Lo ) cells in the spleen of WT and Ikzf3 -/- mice and b corresponding data for percent (%) and counts (#, normalized to 6 × 10 5 events) of CD8 + T VM cells. Data shown for 4 independent experiments, n = 14/group, mean ± SEM, two-sided, unpaired Student’s t-test, **p ≤ 0.01 and ****p ≤ 0.0001. c Frequency (%) and d numbers (#) of T VM cells in the blood (BL), Liver (LV), mediastinal lymph node (mLN) and lung (LG) of WT vs Aiolos-deficient mice (#, normalized to 5 × 105 events each; 3 × 10 5 events for mLN); n = 11 for BL and LV, 2 independent experiments; n = 11 WT, 10 Ikzf3 -/- for mLN, 3 independent experiments; n = 13 WT, 14 Ikzf3 -/- for LG, 4 independent experiments. Data represented as mean ± SEM, two-sided, unpaired Student’s t-test, **p ≤ 0.01, ***p ≤ 0.001, and ****p ≤ 0.0001. Representative histogram overlays and corresponding median fluorescence intensity (MFI) fold change for ( e ) Eomes and ( f ) CD122 in the indicated populations, relative to WT naive CD8 + T cells. Data compiled from 4 independent experiments, n = 14 (for Eomes); and 2 independent experiments, n = 8/group (for CD122). Data presented as mean ± SEM; two-way ANOVA with Tukey’s multiple comparisons test, **p ≤ 0.01 and ****p ≤ 0.0001. Source data are provided as a file.

Article Snippet: Wild-type CD45.2 C57BL/6 J (JAX stock #000664) mice were originally obtained from the Jackson Laboratory.

Techniques: Flow Cytometry, Injection, Labeling, Fluorescence

Journal: Nature Communications

Article Title: Aiolos restricts the generation of antigen-inexperienced, virtual memory CD8 + T cells in mice

doi: 10.1038/s41467-025-67540-8

Figure Lengend Snippet: WT and Aiolos-deficient mice were intranasally (i.n.) infected with 100 plaque-forming units (PFU) of influenza A virus (IAV), and tissues were harvested 1-day post-infection (d.p.i). a Representative flow contour plot depicting resident (CD45.2 (i.v. Neg) ) CD8 + T VM cells in the spleens of WT and Ikzf3 -/- mice 1 d.p.i. and b corresponding percent (%) positive and counts (#, normalized to 6 × 10 5 events) data for CD8 + T VM cells, n = 16 each from 5 independent experiments. c Representative flow plots and d corresponding % and counts (#, normalized to 5 × 105 events) data for resident CD8 + T cells in WT vs. Ikzf3 -/- infected lungs 1 d.p.i. e Frequency (%) and numbers (#, normalized to 5 × 10 5 events) of CD8 + T VM cells in the lungs of WT and Aiolos-deficient mice 1 d.p.i., n = 16 for WT, and 14 for Ikzf3 -/- from 5 independent experiments. For b , d , e , data presented as mean ± SEM; two-sided, unpaired Student’s t-test, ***p ≤ 0.001 and ****p ≤ 0.0001. f Fold change in the number of CD8 + T VM cells in the spleens and lungs of WT and Aiolos-deficient mice 1 d.p.i., relative to WT. Data presented as mean ± SEM; two-sided, unpaired Student’s t-test, **p ≤ 0.01. g Schematic of murine influenza A virus infection and concurrent treatment with Fingolimod (FTY720), created in Microsoft PowerPoint. WT and Ikzf3 -/- mice were intranasally infected with 100 PFU of IAV. Mice were intraperitoneally (i.p.) injected with FTY720 (1 mg/kg/day) or saline control daily starting at 1 day prior (day -1) to infection to 2 days (day 2) post-infection. h Data showing differences in the number (#, normalized to 3 × 105 events) of resident CD8 + T cells in the lungs of WT and Ikzf3 -/- mice in the control treated and FTY720 treated groups. Data shown for 3 independent experiments with n = 10 each, as mean ± SEM, Two-way ANOVA, Tukey’s multiple comparisons test, **p ≤ 0.01 and ****p ≤ 0.0001. Source data are provided as a file.

Article Snippet: Wild-type CD45.2 C57BL/6 J (JAX stock #000664) mice were originally obtained from the Jackson Laboratory.

Techniques: Infection, Virus, Injection, Saline, Control

Journal: Nature Communications

Article Title: Aiolos restricts the generation of antigen-inexperienced, virtual memory CD8 + T cells in mice

doi: 10.1038/s41467-025-67540-8

Figure Lengend Snippet: a Schematic of murine influenza A virus infection and concurrent treatment with Fingolimod (FTY720). Mice were treated with FTY720 (1 mg/kg/day) intraperitoneally (i.p.) at days 1–5 following IAV (100 PFU) infection. Tissues were collected after euthanasia at day 3 or day 6 for flow cytometry and RNA analysis. b Weight of the mice was monitored daily up to 6 days post infection (d.p.i). Data representative of 3 independent experiments with n = 11 WT and 13 Ikzf3 -/- mice, presented as mean ± SEM. *p ≤ 0.05, ****p ≤ 0.0001, two-sided, multiple unpaired Student’s t-tests. c Analysis of frequency (%) and numbers (#, normalized to 3 × 105 events) of CD45.2 (i.v. Neg) CD8 + T cells in the lungs of WT and Ikzf3 -/- mice at day 3 and 6. d qRT-PCR analysis for influenza virus nucleoprotein ( NP ) expression on RNA isolated from the lung homogenates on day 3 and 6. Data normalized to Rps18 and presented relative to WT. For ( c , d ), data are representative of 3 independent experiments, n = 9 each for day 3; n = 11 WT and 13 Ikzf3 -/- for day 6, data presented as mean ± SEM; Two-way ANOVA, Tukey’s multiple comparisons test. **p ≤ 0.01, ***p ≤ 0.001, and ****p ≤ 0.0001. e Schematic of the adoptive transfer of sorted WT and Ikzf3 -/- T VM cells into recipient Cd8 -/- mice and murine influenza virus infection for the determination of viral RNA levels as above. f qRT-PCR analysis for IAV NP on RNA isolated from the lung homogenates on day 3 post-infection. Data normalized to Rps18 and presented relative to WT. Data representative of 4 independent experiments with n = 17 WT and 16 Ikzf3 -/- recipients. Data presented as mean ± SEM; two-sided, unpaired Student’s t-test. *p ≤ 0.05. Schematics in ( a , e ) were created in Microsoft PowerPoint. Source data are provided as a Source Data file.

Article Snippet: Wild-type CD45.2 C57BL/6 J (JAX stock #000664) mice were originally obtained from the Jackson Laboratory.

Techniques: Virus, Infection, Flow Cytometry, Quantitative RT-PCR, Expressing, Isolation, Adoptive Transfer Assay